David M. Koelle, MD
Professor
Department of Medicine, Division of Allergy & Infectious Diseases
Adjunct Professor
Department of Global Health
Joint Professor
Department of Laboratory Medicine & Pathology
Affiliate Investigator
Benaroya Research Institute, Seattle, WA
Affiliate Investigator
Clinical Research Division, Fred Hutchinson Cancer Research Center
Affiliate Investigator
Vaccine & Infectious Diseases Division (VIDD), Fred Hutchinson Cancer Research Center
Attending Physician, Harborview Medical Center
Attending Physician, Virology Clinic
Sites of Practice: 
Harborview Medical Center

Faculty Information

Biography

T-cell immunology is at the core of infectious diseases, cancer, and allergy. We have projects concerning herpes simplex viruses types 1 and 2, varicella zoster virus, vaccinia (the vaccine for smallpox), Merkel cell polyoma virus, and HHV-6, and a project on T cell-mediated severe drug reactions. These pathogens mostly have large genomes, so determining T-cell antigens is challenging. Our technical expertise is various molecular methods to interrogate T-cell responses to a very high level of definition. A suite of modern immunology tools such as intracellular cytokine cytometry, tetramers, T cell cloning, artificial APC, cell killing assays, transgenic TCR expression, etc. are in use to correlate immune variables with outcomes or immune responses to vaccination. HSV-1 and HSV-2 vaccine candidates have been discovered, studied in mice, and entered human trials. We perform immune monitoring for human clinical trials of candidate HSV vaccines. For Mycobacterium tuberculosis (MTB), I am responsible for the IGRA testing at our medical center, and have mentored several trainees interested in this T-cell immunology clinical test. Fellows interested in modern laboratory immunology are welcome. For papers, search Pubmed on "Koelle DM".

Education & Training: 
MD
University of Washington
Seattle WA
1985
Residency
Tufts-New England Medical Center
Boston, MA
1986-1988
Fellowship in Infectious Diseases
University of Washington
Seattle WA
1989-1992
Honors: 
Phi Beta Kappa
1979
Alpha Omega Alpha
1984
Fellow, Infectious Diseases Society of America (IDSA)
2009
American Board of Internal Medicine, Infectious Diseases
2013
American Society for Clinical Investigation
Contact
Phone: 
(206) 616-1940
Mailing Address: 

750 Republican Street, Room E651
Box 358061
Seattle, WA, 98109

 

Research & Clinical Interests
Research Interests: 
  • HSV
  • VZV
  • TB
  • immune testing vaccine clinical trials
  • viral induced cancer
Clinical Interests: 
  • herpesvirus infections
  • viral cancers
  • viral and cancer vaccine clinical trials
  • immune mediated disorders
Publications
Publications: 

Nayak K, Jing L, Russell RM, Davies DH, Hermanson G, Molina DM, Liang X, Sherman DR, Kwok WW, Yang J, Kenneth J, Ahamed SF, Chandele A, Kaja M-K, Koelle DM.  2015. Identification of novel Mycobacterium tuberculosis CD4 T-cell antigens via high throughput proteome scanning. Tuberculosis. 2015 May; 95(3):275-87. Epub 2015 Mar 27.
• PubMed Abstract

Laing KJ, Russell RM, Dong L, Schmid DS, Stern M, Magaret A, Haas J, Johnston C, Wald A, Koelle DM. Zoster vaccination increases the breadth of CD4+ T-cell responses to varicella zoster virus. Journal of Infectious Diseases. 2015 Oct 1;212(7):1022-31.
• PubMed Abstract

Chapuis AG, Afanasiev O, Iyer J, Paulson KG, Parvathaneni U, Hwang JH, Lai I, Roberts IM, Sloan HL, Bhatia S, Shibuya K, Gooley T, Desmarais C, Koelle DM., Yee C, Nghiem P. Regression of metastatic Merkel cell carcinoma following transfer of polyoma-specific T-cells and therapies capable of re-inducing HLA class. Cancer Immunology Research. 2014; 2: 27-36.
• PubMed Abstract

Jing L, Schiffer JT, Chong TM, Bruckner JJ, Davies DH, Felgner PL, Haas J, Wald A, Verjans GM, Koelle DM. CD4 T-cell memory responses to viral infections of humans show pronounced immunodominance independent of duration or viral persistence. J Virol. 2013; 87(5):2617-27.
• PubMed Abstract

van Velzen M, Jing L, Osterhaus ADME, Sette A, Koelle DM*, Verjans GMGM* (*Co-senior author). Local CD4 and CD8 T-cell reactivity to HSV-1 antigens documents broad viral protein expression and immune competence in human ganglia. PLoS Pathogens, 2013;  9:e1003547.
• PubMed Abstract

Jing L, Haas J, Chong TM, Bruckner JJ, Dann GC, Dong L, Marshak JO, McClurkan CL, Yamamoto TN, Bailer SM, Laing KJ, Wald A, Verjans GM, Koelle DM. Cross-presentation and genome-wide screening reveal candidate T cells antigens for a herpes simplex virus type 1 vaccine. J Clin Invest. 2012; 122(2):654-73.
• PubMed Abstract