Jaisri Lingappa, MD, PhD | Allergy and Infectious Diseases

Professor

Department of Global Health

Adjunct Professor

Department of Medicine, Division of Allergy and Infectious Diseases

Adjunct Professor

Department of Microbiology

Faculty Information

Biography

Dr. Lingappa is well known for her studies demonstrating that host enzymes are co-opted by viruses for the purpose of facilitating capsid assembly. In 2002, her lab reported in the journal Nature that a cellular ATPase, ABCE1, plays a critical role in assembly of the HIV-1 viral capsid. More recently, her group demonstrated that the cellular factor DDX6, an RNA helicase, is critical for HIV-1 capsid assembly.

Education & Training:

University of Massachusetts

Boston MA

PhD

Harvard University

Cambridge MA

Honors:

Joe McKee International Red Ribbon Award

2012

Contact

Email:

jais@uw.edu

Phone:

(206) 616-6285

Mailing Address:

Dept. of Global Health
University of Washington
1616 Eastlake Avenue East, Suite 300
Seattle, WA 98102

Research & Clinical Interests

Research Interests:

Jaisri Lingappa’s lab studies how viruses hijack host proteins to promote assembly of human immunodeficiency virus type 1 (HIV-1) and other viruses, using biochemical and cell biological approaches. Our group has demonstrated that, in cells, the assembly of immature HIV-1 capsids occurs through a pathway of assembly intermediates, and is facilitated by the catalytic activity of the host enzymes ABCE1 and DDX6. To form assembly intermediates (also called “assembly machines”), HIV-1 co-opts sites of RNA metabolism called RNA granules. Our most recent studies suggest that:

Publications

PubMed:

PubMed Bibliography

Publications:

Sette, P, O'Connor, SK, Yerramilli, VS, Dussupt, V, Nagashima, K, Chutiraka, K, Lingappa, J, Scarlata, S, Bouamr, F. HIV-1 Nucleocapsid Mimics the Membrane Adaptor Syntenin PDZ to Gain Access to ESCRTs and Promote Virus Budding. Cell Host Microbe. 2016;19 (3):336-48. doi: 10.1016/j.chom.2016.02.004. PubMed PMID:26962944 PubMed Central PMC4804359.

Tanaka, M, Robinson, BA, Chutiraka, K, Geary, CD, Reed, JC, Lingappa, JR. Mutations of Conserved Residues in the Major Homology Region Arrest Assembling HIV-1 Gag as a Membrane-Targeted Intermediate Containing Genomic RNA and Cellular Proteins. J. Virol. 2015;90 (4):1944-63. doi: 10.1128/JVI.02698-15. PubMed PMID:26656702 PubMed Central PMC4734008.

Lingappa, JR, Reed, JC, Tanaka, M, Chutiraka, K, Robinson, BA. How HIV-1 Gag assembles in cells: Putting together pieces of the puzzle. Virus Res. 2014;193 :89-107. doi: 10.1016/j.virusres.2014.07.001. PubMed PMID:25066606 PubMed Central PMC4351045.

Robinson, BA, Reed, JC, Geary, CD, Swain, JV, Lingappa, JR. A temporospatial map that defines specific steps at which critical surfaces in the Gag MA and CA domains act during immature HIV-1 capsid assembly in cells. J. Virol. 2014;88 (10):5718-41. doi: 10.1128/JVI.03609-13. PubMed PMID:24623418 PubMed Central PMC4019110.

Lingappa, UF, Wu, X, Macieik, A, Yu, SF, Atuegbu, A, Corpuz, M, Francis, J, Nichols, C, Calayag, A, Shi, H, Ellison, JA, Harrell, EK, Asundi, V, Lingappa, JR, Prasad, MD, Lipkin, WI, Dey, D, Hurt, CR, Lingappa, VR, Hansen, WJ, Rupprecht, CE. Host-rabies virus protein-protein interactions as druggable antiviral targets. Proc. Natl. Acad. Sci. U.S.A. 2013;110 (10):E861-8. doi: 10.1073/pnas.1210198110. PubMed PMID:23404707 PubMed Central PMC3593902.

Reed, JC, Molter, B, Geary, CD, McNevin, J, McElrath, J, Giri, S, Klein, KC, Lingappa, JR. HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly. J. Cell Biol. 2012;198 (3):439-56. doi: 10.1083/jcb.201111012. PubMed PMID:22851315 PubMed Central PMC3413349.

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