Jaisri Lingappa, MD, PhD
Professor
Department of Global Health
Adjunct Professor
Department of Medicine, Division of Allergy & Infectious Diseases
Adjunct Professor
Department of Microbiology

Faculty Information

Biography

Dr. Lingappa is well known for her studies demonstrating that host enzymes are co-opted by viruses for the purpose of facilitating capsid assembly. In 2002, her lab reported in the journal Nature that a cellular ATPase, ABCE1, plays a critical role in assembly of the HIV-1 viral capsid. More recently, her group demonstrated that the cellular factor DDX6, an RNA helicase, is critical for HIV-1 capsid assembly.

Education & Training: 
MD
University of Massachusetts
Boston MA
PhD
Harvard University
Cambridge MA
Honors: 
Joe McKee International Red Ribbon Award
2012
Contact
Email: 
Phone: 
(206) 616-6285
Mailing Address: 

Department of Global Health
University of Washington
1616 Eastlake Avenue East, Suite 300
Seattle, WA 98102

Research & Clinical Interests
Research Interests: 

Jaisri Lingappa’s lab studies how viruses hijack host proteins to promote assembly of human immunodeficiency virus type 1 (HIV-1) and other viruses, using biochemical and cell biological approaches. Our group has demonstrated that, in cells, the assembly of immature HIV-1 capsids occurs through a pathway of assembly intermediates, and is facilitated by the catalytic activity of the host enzymes ABCE1 and DDX6. To form assembly intermediates (also called “assembly machines”), HIV-1 co-opts sites of RNA metabolism called RNA granules.

Publications
Publications: 

Sette P, O'Connor SK, Yerramilli VS, Dussupt V, Nagashima K, Chutiraka K, Lingappa J, Scarlata S, Boumir F. HIV-1 nucleocapsid mimics the membrane adaptor syntenin PDZ to gain access to ESCRTs and promote virus budding. Cell Host Microbe. 2016; 19(3):336-48.
• PubMed Abstract

Tanaka M, Robinson BA, Chutiraka K, Geaury CD, Reed JC, Lingappa JR. Mutations of conserved residues in the major homology region arrest assembling HIV-1 Gag as a membrane-targeted intermediate containing genomic RNA and cellular proteins. J. Virol. 2015; 90(4):1944-63.
• PubMed Abstract

Lingappa JR, Reed RC, Tanaka M, Chutiraka K, Robinson BA. How HIV-1 Gag assembles in cells: Putting together pieces of the puzzle. Virus Res. 2014; 193:89-107. 
 PubMed Abstract

Robinson BA, Reed JC, Geary CD, Swain JV, Lingappa JR. A temporospatial map that defines specific steps at which critical surfaces in the Gag MA and CA domains act during immature HIV-1 capsid assembly in cells. J. Virol. 2014; 88(10):5718-41.
 PubMed Abstract

Lingappa UF, Wu X, Macieik A, Yu SF, Atuegbu A, Corpuz M, Francis J, Nichols C, Calayag A, Shi H, Ellison JA, Harrell EK, Asundi V, Lingappa JR, Prasad MD, Lipkin WI, Dey D, Hurt CR, Lingappa VR, Hansen WJ, Rupprecht CE. Host-rabies virus protein-protein interactions as druggable antiviral targets. Proc. Natl. Acad. Sci. USA. 2013; 110 (10):E861-8.
 PubMed Abstract

Reed JC, Molter B, Geary CD, McNevin J, McElrath J, Giri S, Klein KC, Lingappa JR. HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly. J. Cell Biol. 2012; 198(3):439-56.
 PubMed Abstract