Karol Bomsztyk, MD
Professor
Department of Medicine, Division of Allergy & Infectious Diseases
Adjunct Professor
Department of Pharmacology
Adjunct Professor
Department of Bioengineering
Attending Physician, UWMC, HMC, SCCA
Sites of Practice: 
Harborview Medical Center
Seattle Cancer Care Alliance
University of Washington Medical Center

Faculty Information

Biography

Detrimental epigenetic processes are emerging as major pathophysiologic culprits of disease including inflammation, infection and organ injury. These processes involve signaling, transcription and epigenetic factors that regulate gene transcription in the chromatin environment, or the epigenome. To better define epigenetic processes in health and disease, our laboratory has been developing high throughput and sensitivity epigenetic platforms and computational tools and have applied them to study acute organ injury, including sepsis. Using these methods and computational tools we have also began series of studies to better understand epigenetic bases of HIV latency.

Current research projects available to trainees include epigenetic studies of inflammation as well as multiple organ injury in experimental sepsis, epigenetic bases of HIV latency, development of high throughput and sensitivity epigenetic methods, development of urine and blood epigenetic biomarkers, development of computational tools to acquire, analyze and visualize epigenetic data.

Research funding: NIH, Life Sciences Discovery Fund (LSDF) of Washington and The Foundation for AIDS Research (amfAR).

Education & Training: 
MD
University of Rochester, School of Medicine
Rochester, NY
1977
Intern in Medicine
Yale-New Haven Hospital
New Haven, CT
1977-1978
Resident in Medicine
Yale-New Haven Hospital
New Haven, CT
1978-1980
Fellow in Nephrology, Department of Medicine
Yale University
New Haven, CT
1980-1983
Honors: 
Established Investigatorship Award, American Heart Association
American Society Clinical Investigator
MERIT Award, NIH
Association of American Physicians
Co-founder Matchstick Technologies Inc. Kirkland WA
Alpha Omega Alpha, University of Rochester
Phi Beta Kappa, Brooklyn College
Summa Cum Laude, Brooklyn College
Contact
Phone: 
(206) 616-7949
Mailing Address: 

850 Republican Street

Seattle, WA 98109

Research & Clinical Interests
Research Interests: 

Development of high throughput technologies and computational tools to reverse engineer epigenetic pathways in health and disease

Clinical Interests: 
Internal medicine
Nephrology
Publications
Publications: 

Bomsztyk K, Mar D, Wang Y, Denisenko O, Ware C, Frazar CD, Blattler A, Maxwell AD, MacConaghy BE, Matula TJ. PIXUL-ChIP: integrated high-throughput sample preparation and analytical platform for epigenetic studies. Nucleic Acids Res. 2019; 47:e69.
• PubMed Abstract

Sharifian R, Okamura DM, Denisenko O, Zager RA, Johnson A, Gharib SA, Bomsztyk K. Distinct patterns of transcriptional and epigenetic alterations characterize acute and chronic kidney injury. Sci Rep. 2018; 8:17870.

• PubMed Abstract

Mikula M, Skrzypczak M, Goryca K, Paczkowska K, Ledwon, JK, Statkiewicz M, Kulecka M, Grzelak M, Dabrowska M, Kuklinska U, Karczmarski J, Rumienczyk I, Jastrzebski K, Miaczynska M, Ginalski K, Bomsztyk K, and Ostrowski J. Genome-wide co-localization of active EGFR and downstream ERK pathway kinases mirrors mitogen-inducible RNA polymerase 2 genomic occupancy, Nucleic Acids Res. 2016; 44:10150-10164.

• PubMed Abstract

Mar D, Gharib SA, Zager RA, Johnson A, Denisenko O, Bomsztyk K. Heterogeneity of epigenetic changes at ischemia/reperfusion- and endotoxin-induced acute kidney injury genes. Kidney Int. 2015; 88:734-744.

• PubMed Abstract

Bomsztyk K, Mar D, An D, Sharifian R, Mikula M, Gharib SA, Altemeier WA, Liles WC, Denisenko O. Experimental acute lung injury induces multi-organ epigenetic modifications in key angiogenic genes implicated in sepsis-associated endothelial dysfunction. Crit Care. 2015 May 11; 19(1):225.
• PubMed Abstract

Reddy MA, Sumanth P, Lanting L, Yuan H, Wang M, Mar D, Alpers CE, Bomsztyk K, Natarajan R. Losartan reverses permissive epigenetic changes in renal glomeruli of diabetic db/db mice. Kidney Int. 2014 Feb; 85(2):362-73.
• PubMed Abstract

Bomsztyk K, Flanagin S, Mar D, Mikula M, Johnson A, Zager R, Denisenko O. Synchronous recruitment of epigenetic modifiers to endotoxin synergistically activated Tnf-α gene in acute kidney injury. PLoS One. 2013 Jul 30; 8(7):e70322.
• PubMed Abstract

Komers R, Mar D, Denisenko O, Xu B, Oyama TT, Bomsztyk K. Epigenetic changes in renal genes dysregulated in mouse and rat models of type 1 diabetes. Lab Invest. 2013 May; 93(5):543-52.
• PubMed Abstract

Yu J, Feng Q, Ruan Y, Komers R, Kiviat N, Bomsztyk K. Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays. BMC Mol Biol. 2011; 12:49.
• PubMed Abstract