Samuel I. Miller, MD
Professor
Professor, Department of Medicine, Division of Allergy & Infectious Diseases
Professor
Department of Microbiology
Professor
Department of Genome Sciences
Adjunct Professor
Department of Immunology University of Washington

Faculty Information

Biography

Dr. Miller holds a B.A. from Johns Hopkins University and a M.D. from Baylor College of Medicine. Dr. Miller is a former faculty member of the Harvard Medical School. In addition to his Microbiology Department appointment, he is a Professor in the Department of Medicine, Division of Allergy and Infectious Diseases, and in the Department of Genome Sciences. He is an Adjunct Professor of Immunology. He is a past Director of a Research Center of Excellence in Biodefense and Emerging Infectious Diseases for the region and Director of the Cystic Fibrosis Research and Development Program at the University of Washington.

Education & Training: 
BA
John Hopkins University
1975
MD
Baylor College of Medicine
1979
Contact
Phone: 
(206) 616-5110
Mailing Address: 

1959 NE Pacific St.

Box 357710

Seattle, WA 98195

Research & Clinical Interests
Research Interests: 
  • Salmonellae translocated effectors which are delivered across the phagosome membrane and function to modify host processes by binding host GTPases, and acting as glycerol-cholesterol acyltransferases and ubiquitin ligases.
  • Bioinformatic analysis, DNA sequencing and phenotypic comparison of Shigella and Salmonella outbreak strains from diverse geographic locations including Central America and Africa and specifically the emergence of non-typhoidal Salmonella strains in Africa.
  • The environmental remodeling of the gram-negative bacterial envelope that occurs when bacteria infect host tissues and specifically the regulated alteration of glycerolphospholipids in the bacterial outer membrane by using a combination of bacterial genetics and mass spectrometry analysis of lipids.
  • Using NMR and crystallography to define structural mechanisms by which PhoQ responds to antimicrobial peptides and low pH and specifically testing the hypothesis that signal transduction involves the loss of structure on one side of the membrane with specific gain of structure on the other side of the membrane.
  • Identification of human genome polymorphisms that alter susceptibility to bacterial infection and diversity of human immune responses by using the human cellular genome wide association study (GWAS) platform using bacteria and the HapMap cell collection of normal human diversity.
  • How the bacterial second messenger di-c-GMP controls cellular processes and use of a FRET based biosensor for c-di-GMP to understand bacterial cellular  function and diversity, and development of compounds that alter human interferon signaling by competing with mammalian molecules that bind cyclic nucleotides.
  • Human studies that involve analysis of the microbiota.  The diseases analyzed using a metagenomic approach and DNA sequencing include inflammatory bowl disease and cystic fibrosis, as well as stool transplantation.
Publications
Publications: 

Mills E, Petersen E, Kulasekara BR, Miller SI, A direct screen for c-di-GMP modulators reveals a Salmonella Typhimurium periplasmic L-arginine-sensing pathway;Science Signaling. 2015 Jun 9; 8(380): ra57. 
• PubMed Abstract

Dalebroux ZD, Edrozo MB, Pfuetzner RA, Ressl S, Kulasekara BR, Blanc MP, Miller SI; Delivery of cardiolipins to the Salmonella outer membrane is necessary for survival within host tissues and virulence; Cell host & microbe. 2015 Apr; 17 4: 441-51.
• PubMed Abstract

Matamouros S, Hayden HS, Hager KR, Brittnacher MJ, Lachance K, Weiss EJ, Pope CE, Imhaus AF, McNally CP, Borenstein E, Hoffman LR, Miller SI. Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis. Proc Natl Acad Sci USA. 2018 Feb 13; 115(7):1605-1610.
• PubMed Abstract

Chaudhary A, Leite M, Kulasekara BR, Altura MA, Ogahara C, Weiss E, Fu W, Blanc MP, O'Keeffe M, Terhorst C, Akey JM, Miller SI. Human Diversity in a Cell Surface Receptor that Inhibits Autophagy. Curr Biol. 2016 Jul 25; 26(14):1791-801.
• PubMed Abstract