James I.

Mullins

PhD

related links

bio

The Mullins laboratory uses the techniques of molecular, computational, and virus biology to provide basic insights into the HIV-human host relationship. Our goals are to assist the fight against AIDS by gaining insight into the establishment of infection and the development of the disease in order to assist creation of effective vaccines and to refine therapies. Current effort utilizes a variety of techniques to define and understand the implications of HIV's extraordinary evolutionary rate and ensuing genetic diversity as well as testing new approaches to vaccine immunogen and therapeutics design. These techniques include virology, molecular biological and statistical analysis of nucleotide sequences. Collaborators include Drs. McElrath, Collier, Horton, Mittler, Zhu, Gottlieb, Campbell, Lingappa, Corey and Celum.

research interests

• HIV, vaccines
• Cure-related research

publications

Mackelprang, R.D., Carrington, M., Thomas, K.K., Hughes, J.P., Baeten, J.M., Wald, A., Farquhar, C., Fife, K., Campbell, M.S., Kapiga, S., Gao, X., Mullins, J.I., and Lingappa, J.R., Host Genetic and Viral Determinants of Hiv-1 Rna Set Point among Hiv-1 Seroconverters from Sub-Saharan Africa, J Virol 89. 2015; (4):2104-11
• PubMed Abstract

Edlefsen, P.T., Rolland, M., Hertz, T., Tovanabutra, S., Gartland, A.J., Decamp, A.C., Magaret, C.A., Ahmed, H., Gottardo, R., Juraska, M., Mccoy, C., Larsen, B.B., Sanders-Buell, E., Carrico, C., Menis, S., Bose, M., Team, R.V.S., Arroyo, M.A., O'connell, R.J., Nitayaphan, S., Pitisuttithum, P., Kaewkungwal, J., Rerks-Ngarm, S., Robb, M.L., Kirys, T., Georgiev, I.S., Kwong, P.D., Scheffler, K., Pond, S.L., Carlson, J.M., Michael, N.L., Schief, W.R., Mullins, J.I., Kim, J.H., and Gilbert, P.B., Comprehensive Sieve Analysis of Breakthrough Hiv-1 Sequences in the Rv144 Vaccine Efficacy Trial, PLoS Comput Biol. 2015; 11(2):e1003973.
• PubMed Abstract

Wagner, T.A., Mclaughlin, S., Garg, K., Cheung, C.Y., Larsen, B.B., Styrchak, S., Huang, H.C., Edlefsen, P.T., Mullins, J.I., and Frenkel, L.M., Hiv Latency. Proliferation of Cells with Hiv Integrated into Cancer Genes Contributes to Persistent Infection, Science.  2014; 345(6196): 570-3.
• PubMed Abstract

Sunshine J, Kim M, Carlson, JM, Heckerman D, Czartoski J, Migueles SA, Maenza J, Mcelrath MJ, Mullins JI, and Frahm N. Increased sequence coverage through combined targeting of variant and conserved epitopes correlates with control of HIV replication. J Virol. 2014; 88(2):1354-65
• PubMed Abstract

Melhem NM, Smith KN, Huang XL, Colleton BA, Jiang W, Mailliard RB, Mullins JI, and Rinaldo CR. The impact of viral evolution and frequency of variant epitopes on primary and memory human immunodeficiency virus Type 1-Specific Cd8(+) T Cell responses. Virology. 2014; 450-451:34-48.
• PubMed Abstract

Manocheewa S, Lanxon-Cookson EC, Liu Y, Swain JV, Rao U, Maust B, Sunshine JE, Kim M, Rolland M, and Mullins JI, Pairwise growth competition assay for determining the replication fitness of human immunodeficiency viruses, Journal of Visualized Experiments. 2014; 99:e52610.
• PubMed Abstract

Liu Y, Rao U, Mcclure J, Konopa P, Manocheewa S, Kim M, Chen L, Troyer RM, Tebit DM, Holte S, Arts EJ, and Mullins JI. Impact of mutations in highly conserved amino acids of the HIV-1 Gag-P24 and Env-Gp120 proteins on viral replication in different genetic backgrounds. PLoS One. 2014; 9(4):e94240.
• PubMed Abstract

Kulkarni V, Valentin A, Rosati M, Rolland M, Mullins JI, Pavlakis GN, and Felber BK. HIV-1 conserved elements P24ce DNA vaccine induces humoral immune responses with broad epitope recognition in macaques, PLoS One 9. 2014; 10:e111085.
• PubMed Abstract

Kulkarni V, Valentin A, Rosati M, Alicea C, Singh AK, Jalah R, Broderick KE, Sardesai NY, Le Gall S, Mothe B, Brander C, Rolland M, Mullins JI, Pavlakis GN, and Felber BK. Altered response hierarchy and increased T-Cell breadth upon HIV-1 conserved element DNA vaccination in macaques, PLoS One. 2014; 9(1):e86254.
• PubMed Abstract